Immunodetection of Fasciola gigantica Circulating Antigen in Sera of 1 Infected Individuals for Laboratory Diagnosis of Human
نویسندگان
چکیده
3 Abdelfattah M. Attallah, Faisal A. Bughdadi, Atef M. El-Shazly and 4 Hisham Ismail, 5 Research & Development Dept., Biotechnology Research Center, New Damietta 34517, 6 Egypt 7 Biological Sciences Dept., University College, Umm Al-Qura University, Makkah 8 21955, Saudi Arabia 9 Parasitology Dept., Faculty of Medicine, Mansoura University, Mansoura 35516, 10 Egypt 11 12 Running title: A 27-kDa F. gigantica antigen in human serum 13 14 *Correspondence: 15 Dr. Hisham Ismail, 16 R & D Dept., Biotechnology Research Center, 17 PO Box 14, 23 July St, Industrial Zone, New Damietta 34517, 18 Egypt. 19 Tel.: +20572402889 ; Fax: +20572401889 20 E-mail: [email protected] 21 Present address: 22 Dr. Hisham Ismail, Biological Sciences Dept., Umm Al-Qura University, 23 PO Box 2064, Makkah 21955, Saudi Arabia. 24 Tel.: +966566653424 ; Fax: +96625586531 25 E-mail: [email protected] 26 CVI Accepts, published online ahead of print on 14 August 2013 Clin. Vaccine Immunol. doi:10.1128/CVI.00305-13 Copyright © 2013, American Society for Microbiology. All Rights Reserved.
منابع مشابه
Immunodetection of Fasciola gigantica circulating antigen in sera of infected individuals for laboratory diagnosis of human fascioliasis.
Currently, the laboratory diagnosis of human fascioliasis is based on the parasitological examination of parasite eggs in stool specimens and serological detection of specific antibodies in serum samples, which are often unreliable diagnostic approaches. Ideally, a sensitive and specific diagnostic test for Fasciola infection should be based on the detection of circulating Fasciola antigen, whi...
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The purpose of the present study was to evaluate the potential role of the 27-Kilodalton (KDa) antigen versus Fasciola gigantica adult worm regurge antigens in a DOT-Blot assay and to assess this assay as a practical tool for diagnosis fascioliasis in Egyptian patients. Fasciola gigantica antigen of an approximate molecular mass 27-(KDa) was obtained from adult worms by a simple elution SDS-PAG...
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Background & Aim: Fascioliasis as a zoonosis is one of the important parasitic diseases which is caused when fasciola species enter the host’s body. Approximately 2.4 million people throughout the world are infected with this parasite and 180 million people are vulnerable to it. On time diagnosis and rapid treatment of the disease can substantially prevent the harmful effects of the infecti...
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